Journal: Blood

Article Title: High endothelial venules as traffic control points maintaining lymphocyte population homeostasis in lymph nodes.

doi: 10.1182/blood-2011-07-367409

Figure Lengend Snippet: Figure 2. HEV pockets are plastic structures. (A) The right panel in the diagram represents a situation in which lymphocytes are not engulfed within pockets but simply migrating between 2 ECs while the left panel represents lymphocytes present in a true structured pocket underneath the HEV. L Lymphocyte. (B) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ICAM-1 (red) and sytox 63/nuclei (blue) Arrowheads indicate the inner membrane of the EC directly in contact with lymphocytes. (C) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ERTR-7 (red), Desmin (white) and sytox 63/nuclei (blue).Arrows indicate the membranes of myofibroblasts/pericytes that ensheath the conduit system. Data are representative of 3 different experiments (2 mice per experiment).

Article Snippet: 6115BLOOD, 1 DECEMBER 2011 VOLUME 118, NUMBER 23 D ow nloaded from http://ashpublications.org/blood/article-pdf/118/23/6115/1344964/zh804911006115.pdf by guest on 24 February 2025 ERTR-7 and Desmin antibodies were purchased from Acris Antibodies.

Techniques: Ubiquitin Proteomics, Staining, Membrane

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: Vitamin D3 mitigates myopathy and metabolic dysfunction in rats with metabolic syndrome: the potential role of dipeptidyl peptidase-4

doi: 10.1007/s00210-024-03439-3

Figure Lengend Snippet: The effect of 6 weeks treatment with vitamin D3 (10 µg/kg/day, gavage), vildagliptin (Vilda, 10 mg/kg/day, gavage), or combination on metabolic syndrome (MetS)-induced myopathy expressed as soleus muscle advanced glycation end products (AGEs, a ), reactive oxygen species (ROS, b ), nicotinamide adenine dinucleotide phosphate oxidase (NADPH Oxidase, c ), and caspase-3 activity ( d ). MetS was induced by feeding rats 3% salt and 10% fructose for 12 weeks. Values are presented as mean ± SEM ( n = 6/group). One-way ANOVA, followed by Tukey’s test for multiple comparisons, was used for analysis, a vs control, b vs MetS, and. c vs vildagliptin at p < 0.05 . Panel e represents photomicrographs of immunohistochemical staining of desmin in the longitudinal section of rat soleus muscle sections from study groups (n = 3/group) (Scale bar 50 µm × 400)

Article Snippet: Subsequently, the primary rabbit polyclonal antibody for desmin (1:200, # 602–300, AbboMax, San Jose, USA) was applied to the prepared sections, and they were incubated at 4 °C overnight.

Techniques: Activity Assay, Control, Immunohistochemical staining, Staining